The use of affinity column
Advanced affinity columns can be fabricated by binding Protein-A, which specifically interacts with IgG, to SPM.
In comparison with a commercially available protein A (Column-1) column packed with particles and a SPM column, a large number of analytes pass through the Column-1 at the lowered flow rate of a mobile phase, resulting in incomplete separation. While the SPM column can transfer the solution at less than one fifth pressure compared to the commercially available columns. Even at more than ten times faster than the normal flow rate (1.0 mL/min), efficient separation selectivity and the high recovery rate were achieved with the SPM column.
Comparison of column pressure with soft sepharose particles
Column size：4.6 mm i.d. x 50 mm length.
The high flow rate of 10 mL/ min was achieved using the SPM column packed with the particles that keep the pressure below 4 MPa. Meanwhile, with the sepharose 6B (45 – 165 µm) column, the immediate increase in pressure was observed at the flow rate of more than 4 mL/min as particles are soft.