Advanced affinity columns can be fabricated by immobilizing Protein-A, which specifically interacts with IgG, onto SPM.

Compared with a commercially available protein A (Column-1) column packed with particles, the SPM column enables more efficient separation. In the particle-packed column, a large amount of analyte passes through at the reduced flow rate of a mobile phase, resulting in incomplete separation. In contrast, the SPM column can transfer solution at less than one-fifth pressure required for conventional columns. Even at flow rates more than ten times higher than the normal flow rate (1.0 mL/min), the SPM column maintains efficient separation selectivity and recovery.